Phenomenon of hyperexpression of rybosomiccistrons in bone marrow hemopoietic elements in bronchialasthma

Abstract

Aim. To study expression of ribosomic cistrons (RC) of bone marrow hemopoietic elements (BMHE) in bronchial asthma (BA). Material and methods. A total of 37 bronchial asthma (BA) patients were examined. Of them, 20 ones had allergic BA (ABA) and 10 - nonallergic BA (NABA). Seven patients received systemic glucocorticoid hormones (SGH). Control included cells of bone marrow from 7 healthy donors. Transcription and maturation of pre-rRNA in BMHE were analysed using bone marrow samples obtained during the sternal puncture (a modified method of Howell and Black). Results. RC activity was high. It depended on BA variant and the disease phase. In ABA high RC activity persisted in remission. GCH suppress RC activity rise. The maximal effect of GCH suppressive action was seen in myeloid lineage, minimal - in erythroid one. The effect is absent in more mature erythroid cells and bone marrow lymphocytes. Conclusion. The phenomenon of RC activity rise in myeloid, erythroid and lymphoid lineages of the bone marrow of BA patients may reflect contribution of the bone marrow to formation of BA pathogenesis.

Keywords

• bronchial asthma
• bone marrow
• myeloid
• erythroid
• lymphoid lineages
• ribosomic cistrons