Quantitative analysis of erythromycin, its major metabolite and clarithromycin in chicken tissues and eggs via QuEChERS extraction coupled with ultrahigh-performance liquid chromatography-tandem mass spectrometry

Bo Wang; Yali Zhu; Shuyu Liu; Han Zhang; Tianzhu Guan; Xuechao Xu; Xiangfeng Zheng; Zhenquan Yang; Tao Zhang; Genxi Zhang; Kaizhou Xie

Food Chemistry: X (Jun 2024)

Quantitative analysis of erythromycin, its major metabolite and clarithromycin in chicken tissues and eggs via QuEChERS extraction coupled with ultrahigh-performance liquid chromatography-tandem mass spectrometry

Bo Wang,
Yali Zhu,
Shuyu Liu,
Han Zhang,
Tianzhu Guan,
Xuechao Xu,
Xiangfeng Zheng,
Zhenquan Yang,
Tao Zhang,
Genxi Zhang,
Kaizhou Xie

Affiliations

Bo Wang: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China
Yali Zhu: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
Shuyu Liu: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
Han Zhang: School of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China
Tianzhu Guan: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China
Xuechao Xu: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China
Xiangfeng Zheng: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China
Zhenquan Yang: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China; Corresponding author at: College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
Tao Zhang: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
Genxi Zhang: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China
Kaizhou Xie: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China; Corresponding author at: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Journal volume & issue: Vol. 22
p. 101468

Abstract

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A simple, rapid and novel method involving ultrahigh-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (UHPLC-ESI–MS/MS) was developed to simultaneously detect erythromycin, its major metabolite and clarithromycin in chicken tissues (muscle, liver and kidney) and eggs (whole egg, albumen and yolk). Samples were extracted using acetonitrile–water (80:20, v/v), and a Cleanert MAS-Q cartridge was used to perform quick, easy, cheap, effective, rugged, and safe (QuEChERS) purification. The average recoveries were 87.78–104.22 %, and the corresponding intraday and interday relative standard deviations were less than 7.10 %. The decision limits and detection capabilities of the chicken tissues and eggs were 2.15–105.21 μg/kg and 2.26–110.42 μg/kg, respectively. For chicken tissues and eggs, the limits of detection and limits of quantification were 0.5 μg/kg and 2.0 μg/kg, respectively. The proposed method was successfully employed to analyse real samples, demonstrating its applicability.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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