An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells

Abstract

Summary: Inducible biomolecular condensates play fundamental roles in cellular responses to intracellular and environmental cues. Knowledge about their composition is crucial to understand the functions that arise specifically from the assembly of condensates. This protocol combines an optogenetic and an efficient proximity labeling approach to analyze protein modifications driven by protein condensation in cultured cells. Low endogenous biotin level ensures sharp signals.For complete details on the use and execution of this protocol, please refer to Frattini et al. (2021).

Keywords

• Biophysics
• Cell Biology
• Microscopy
• Molecular/Chemical Probes
• Protein Biochemistry