Cancer Reports (Jun 2024)
LncRNA PVT1 facilitates the growth and metastasis of colorectal cancer by sponging with miR‐3619‐5p to regulate TRIM29 expression
Abstract
Abstract Background Colorectal cancer (CRC) is the second most common cause of cancer‐related death worldwide. Long noncoding RNA (lncRNA) is involved in many malignant tumors. This study aimed to clarify the role of the lncRNA plasmacytoma variant translocation 1 (PVT1) in CRC growth and metastasis. Methods Differentially expressed lncRNAs in CRC were analyzed using the Cancer Genome Atlas. Gene expression profiling interactive analysis and a comprehensive resource for lncRNAs from cancer arrays databases were used to analyze lncRNA PVT1 expression and CRC prognosis, respectively. Cell counting kit‐8, wound healing, colony formation, Transwell, and immunofluorescence assays were used to evaluate CRC cell proliferation, migration, invasion, and epithelial‐mesenchymal transition (EMT), respectively. Tumor growth and metastasis models were used to explore the PVT1 effect on the growth and metastasis of CRC in vivo. Results PVT1 was highly expressed in CRC, associated with a poor prognosis of CRC, and showed good diagnostic value. Transfection of sh‐PVT1 or pcDNA3.1‐PVT1 reduced or increased the proliferation, wound healing rate, colony formation, invasion, and EMT of CRC cells. PVT1 and miR‐3619‐5p were co‐expressed in CRC cytoplasm, and PVT1 acted as a competitive endogenous RNA (ceRNA) by sponging miR‐3619‐5p to up‐regulate tripartite motif containing 29 (TRIM29) expression. MiR‐3619‐5p overexpression and TRIM29 knockdown reduced proliferation, wound healing rate, invasion, and EMT of CRC cells. However, simultaneous PVT1 and miR‐3619‐5p overexpression or knockdown of miR‐3619‐5p and TRIM29 knockdown rescued the malignant phenotype of CRC cells. Conclusions We first clarified the ceRNA mechanism of PVT1 in CRC, which induced growth and metastasis by sponging with miR‐3619‐5p to regulate TRIM29.
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