Nature Communications (Nov 2020)
BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
- Michael Zabolocki,
- Kasandra McCormack,
- Mark van den Hurk,
- Bridget Milky,
- Andrew P. Shoubridge,
- Robert Adams,
- Jenne Tran,
- Anita Mahadevan-Jansen,
- Philipp Reineck,
- Jacob Thomas,
- Mark R. Hutchinson,
- Carmen K. H. Mak,
- Adam Añonuevo,
- Leon H. Chew,
- Adam J. Hirst,
- Vivian M. Lee,
- Erin Knock,
- Cedric Bardy
Affiliations
- Michael Zabolocki
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- Kasandra McCormack
- STEMCELL Technologies
- Mark van den Hurk
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- Bridget Milky
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- Andrew P. Shoubridge
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- Robert Adams
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- Jenne Tran
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- Anita Mahadevan-Jansen
- Vanderbilt Biophotonics Centre, Vanderbilt University
- Philipp Reineck
- ARC Centre of Excellence for Nanoscale BioPhotonics & School of Science, RMIT University
- Jacob Thomas
- Adelaide Medical School, University of Adelaide
- Mark R. Hutchinson
- Adelaide Medical School, University of Adelaide
- Carmen K. H. Mak
- STEMCELL Technologies
- Adam Añonuevo
- STEMCELL Technologies
- Leon H. Chew
- STEMCELL Technologies
- Adam J. Hirst
- STEMCELL Technologies
- Vivian M. Lee
- STEMCELL Technologies
- Erin Knock
- STEMCELL Technologies
- Cedric Bardy
- Laboratory for Human Neurophysiology and Genetics, South Australian Health and Medical Research Institute (SAHMRI)
- DOI
- https://doi.org/10.1038/s41467-020-19275-x
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 19
Abstract
Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.
