Проблемы особо опасных инфекций (Jun 2010)

Obtaining of Polyclonal Antibodies against <i>B. anthracis</i> S-layer Proteins and Study of their Specificity in Enzyme Immunoassay

  • A. Yu. Goncharova,
  • N. I. Mikshis,
  • O. M. Kudryavtseva,
  • M. F. Bolotnikova,
  • L. V. Novikova,
  • G. G. Krasichkov,
  • T. V. Alenkina,
  • Yu. A. Popov

DOI
https://doi.org/10.21055/0370-1069-2010-3(105)-46-49
Journal volume & issue
Vol. 0, no. 3(105)
pp. 46 – 49

Abstract

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Purified preparations of B. anthracis S-layer proteins - Sap and EA1 were obtained. Developed was the scheme of laboratory animals` immunization, that enabled to receive rabbits' polyclonal antibodies against Sap and EA1 proteins in high titer. 12 strains of B. anthracis, 53 strains of closely-related bacilli and 4 strains of other heterogeneous bacteria were tested in enzyme-linked immunosorbent assay and DOT-immunoassay with the use of polyclonal anti-Sap and anti-EA1 antibodies. Analysis of specificity of the obtained antibodies revealed cross-reactions with B. cereus, B. subtilis, B. mycoides and B. megaterium. The specificity of polyclonal anti-EA1 and anti-Sap antibodies was 75 and 83 % respectively. Sensitivity of reaction was 105 and106 m.c. in applying polyclonal antibodies against B. anthracis S-layer proteins

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