Validation and performance comparison of two SARS-CoV-2 IgG/IgM rapid tests

Nisreen Naser Al Awaji; Hanadi Talal Ahmedah; Isra Mohammed Alsaady; Rowaedh Ahmed Bawaked; Mohammed A. Alraey; Ahmed Ayed Alasiri; Abdullah Mofareh Alfaifi; Hamdan Ali Alshehri; Raed Alserihi; Elrashed B. Yasin

Saudi Journal of Biological Sciences (Jun 2021)

Validation and performance comparison of two SARS-CoV-2 IgG/IgM rapid tests

Nisreen Naser Al Awaji,
Hanadi Talal Ahmedah,
Isra Mohammed Alsaady,
Rowaedh Ahmed Bawaked,
Mohammed A. Alraey,
Ahmed Ayed Alasiri,
Abdullah Mofareh Alfaifi,
Hamdan Ali Alshehri,
Raed Alserihi,
Elrashed B. Yasin

Affiliations

Nisreen Naser Al Awaji: Department of Health Communication Sciences, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, P.O Box 84428, Riyadh 11671, Saudi Arabia
Hanadi Talal Ahmedah: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Rabegh, Saudi Arabia; Corresponding author at: King Abdulaziz University, Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, Rabigh, Saudi Arabia.
Isra Mohammed Alsaady: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Special Infectuis Agent unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
Rowaedh Ahmed Bawaked: Centre of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia
Mohammed A. Alraey: Infection Prevention and Control Department, King Abdullah bin Abdulaziz University Hospital, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia
Ahmed Ayed Alasiri: Department of Pathology and Laboratory Medicine, Prince Mohammed Bin Abdulaziz Hospital, Riyadh, Saudi Arabia
Abdullah Mofareh Alfaifi: Department of Pathology and Laboratory Medicine, King Abdullah Bin Abdulaziz University Hospital, Riyadh, Saudi Arabia
Hamdan Ali Alshehri: Department of Pathology and Clinical Laboratory, King Abdullah Bin Abdulaziz University Hospital, Riyadh, Saudi Arabia
Raed Alserihi: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
Elrashed B. Yasin: Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Rabigh, Saudi Arabia

Journal volume & issue: Vol. 28, no. 6
pp. 3433 – 3437

Abstract

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a disease called COVID-19. COVID-19 is primarily diagnosed using molecular techniques mainly real-time reverse transcriptase PCR. Reliable and accurate serologic assays for COVID-19, are an important tool for surveillance and epidemiologic studies. In this study, the IgG/IgM Rapid Test Cassette and the Prima COVID-19 IgG/IgM Rapid Test for the detection of SARS-CoV-2 antibodies in blood, serum and plasma samples collected from patients up to 48 days after symptom onset in Saudi Arabia were validated. Overall, both tests showed poor performance and cannot be utilised for COVID-19 diagnosis as a point of care test or to determine seroprevalence.

Published in Saudi Journal of Biological Sciences

ISSN: 1319-562X (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Science: Biology (General)
Website: http://www.journals.elsevier.com/saudi-journal-of-biological-sciences/

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