Analysis of an Alanine/Arginine Mixture by Using TLC/FTIR Technique

Jun Liu; Feng-shan Zhou; Ran Guo; Ye Jiang; Xiaokun Fan; Anqi He; Yanjun Zhai; Shifu Weng; Zhanlan Yang; Yizhuang Xu; Isao Noda; Jinguang Wu

doi:10.1155/2014/925705

Journal of Spectroscopy (Jan 2014)

Analysis of an Alanine/Arginine Mixture by Using TLC/FTIR Technique

Jun Liu,
Feng-shan Zhou,
Ran Guo,
Ye Jiang,
Xiaokun Fan,
Anqi He,
Yanjun Zhai,
Shifu Weng,
Zhanlan Yang,
Yizhuang Xu,
Isao Noda,
Jinguang Wu

Affiliations

Jun Liu: Department of Biochemistry, Wenshan University, Wenshan 663000, China
Feng-shan Zhou: School of Materials Science and Technology, China University of Geosciences, Beijing 100083, China
Ran Guo: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Ye Jiang: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Xiaokun Fan: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Anqi He: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Yanjun Zhai: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang 11660, China
Shifu Weng: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Zhanlan Yang: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Yizhuang Xu: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Isao Noda: Department of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA
Jinguang Wu: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

DOI: https://doi.org/10.1155/2014/925705
Journal volume & issue: Vol. 2014

Abstract

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We applied TLC/FTIR coupled with mapping technique to analyze an alanine/arginine mixture. Narrow band TLC plates prepared by using AgI as a stationary phase were used to separate alanine and arginine. The distribution of alanine and arginine spots was manifested by a 3D chromatogram. Alanine and arginine can be successfully separated by the narrow band TLC plate. In addition, the FTIR spectra of the separated alanine and arginine spots on the narrow band TLC plate are roughly the same as the corresponding reference IR spectra.

Published in Journal of Spectroscopy

ISSN: 2314-4920 (Print); 2314-4939 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Physics: Optics. Light
Website: https://onlinelibrary.wiley.com/journal/9169

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