AttentionEP: Predicting essential proteins via fusion of multiscale features by attention mechanisms

Abstract

Identifying essential proteins is of utmost importance in the field of biomedical research due to their essential functions in cellular activities and their involvement in mechanisms related to diseases. In this research, a novel approach called AttentionEP for predicting essential proteins (EP) is introduced by attention mechanisms. This method leverages both cross-attention and self-attention frameworks, focusing on enhancing prediction accuracy through the integration of features across diverse scales. Spatial characteristics of proteins are obtained from the protein-protein interaction (PPI) network by employing Graph Convolutional Networks (GCN) and Graph Attention Networks (GAT). Following this, Bidirectional Long Short-Term Memory networks (BiLSTM) are employed to derive temporal features from gene expression datasets. Furthermore, spatial characteristics are derived by integrating data on subcellular localization with the application of Deep Neural Networks (DNN). In order to effectively integrate features across multiple scales, initial steps involve the application of self-attention techniques to derive essential insights from each unique data set. Following this, mechanisms involving self-attention and cross-attention are employed to enhance the interaction between diverse information sources. To identify essential proteins, a classifier based on the ResNet architecture is developed. The findings from the experiments indicate that the method introduced here shows superior performance in identifying essential proteins, recording an Area Under the Curve (AUC) value of 0.9433. This approach shows a considerable advantage over established techniques. The findings of this study provide a significant advancement in the comprehension of critical proteins, revealing promising potential for applications in the development of therapeutics and addressing various diseases.

Keywords

• Essential protein prediction
• Feature fusion
• Deep learning
• Attention mechanism