Frontiers in Microbiology (Jan 2024)
Identification of L11L and L7L as virulence-related genes in the African swine fever virus genome
- Jiaqi Fan,
- Jiaqi Fan,
- Jiaqi Fan,
- Jingyuan Zhang,
- Fengjie Wang,
- Fengjie Wang,
- Fengjie Wang,
- Faming Miao,
- Faming Miao,
- Han Zhang,
- Han Zhang,
- Yiqian Jiang,
- Yiqian Jiang,
- Yu Qi,
- Yu Qi,
- Yanyan Zhang,
- Yanyan Zhang,
- Lili Hui,
- Lili Hui,
- Dan Zhang,
- Dan Zhang,
- Huixian Yue,
- Huixian Yue,
- Xintao Zhou,
- Xintao Zhou,
- Xintao Zhou,
- Qixuan Li,
- Qixuan Li,
- Yu Wang,
- Yu Wang,
- Teng Chen,
- Teng Chen,
- Rongliang Hu,
- Rongliang Hu,
- Rongliang Hu
Affiliations
- Jiaqi Fan
- College of Life Sciences, Ningxia University, Yinchuan, Ningxia, China
- Jiaqi Fan
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Jiaqi Fan
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Jingyuan Zhang
- Institute of Rare Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan, China
- Fengjie Wang
- College of Life Sciences, Ningxia University, Yinchuan, Ningxia, China
- Fengjie Wang
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Fengjie Wang
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Faming Miao
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Faming Miao
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Han Zhang
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Han Zhang
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Yiqian Jiang
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Yiqian Jiang
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Yu Qi
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Yu Qi
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Yanyan Zhang
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Yanyan Zhang
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Lili Hui
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Lili Hui
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Dan Zhang
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Dan Zhang
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Huixian Yue
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Huixian Yue
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Xintao Zhou
- College of Life Sciences, Ningxia University, Yinchuan, Ningxia, China
- Xintao Zhou
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Xintao Zhou
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Qixuan Li
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Qixuan Li
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Yu Wang
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Yu Wang
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Teng Chen
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Teng Chen
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- Rongliang Hu
- College of Life Sciences, Ningxia University, Yinchuan, Ningxia, China
- Rongliang Hu
- Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Ministry of Agriculture and Rural Affairs, Changchun, Jilin, China
- Rongliang Hu
- Chinese Academy of Agricultural Sciences Changchun Veterinary Research Institute, Changchun, Jilin, China
- DOI
- https://doi.org/10.3389/fmicb.2024.1345236
- Journal volume & issue
-
Vol. 15
Abstract
IntroductionAfrican swine fever (ASF) is an infectious disease that causes considerable economic losses in pig farming. The agent of this disease, African swine fever virus (ASFV), is a double-stranded DNA virus with a capsid membrane and a genome that is 170-194 kb in length encoding over 150 proteins. In recent years, several live attenuated strains of ASFV have been studied as vaccine candidates, including the SY18ΔL7-11. This strain features deletion of L7L, L8L, L9R, L10L and L11L genes and was found to exhibit significantly reduced pathogenicity in pigs, suggesting that these five genes play key roles in virulence.MethodsHere, we constructed and evaluated the virulence of ASFV mutations with SY18ΔL7, SY18ΔL8, SY18ΔL9, SY18ΔL10, and SY18ΔL11L.ResultsOur findings did not reveal any significant differences in replication efficiency between the single-gene deletion strains and the parental strains. Pigs inoculated with SY18ΔL8L, SY18ΔL9R and SY18ΔL10L exhibited clinical signs similar to those inoculated with the parental strains. Survival rate of pigs inoculated with 103.0TCID50 of SY18ΔL7L was 25%, while all pigs inoculated with 103.0TCID50 of SY18ΔL11L survived, and 50% inoculated with 106.0TCID50 SY18ΔL11L survived.DiscussionThe results indicate that L8L, L9R and L10L do not affect ASFV SY18 virulence, while the L7L and L11L are associated with virulence.
Keywords
