Chromosome Level Genome Assembly of Andrographis paniculata

Ying Liang; Shanshan Chen; Kunhua Wei; Zijiang Yang; Shengchang Duan; Yuan Du; Peng Qu; Jianhua Miao; Wei Chen; Wei Chen; Wei Chen; Yang Dong; Yang Dong; Yang Dong; Yang Dong

doi:10.3389/fgene.2020.00701

Frontiers in Genetics (Jun 2020)

Chromosome Level Genome Assembly of Andrographis paniculata

Ying Liang,
Shanshan Chen,
Kunhua Wei,
Zijiang Yang,
Shengchang Duan,
Yuan Du,
Peng Qu,
Jianhua Miao,
Wei Chen,
Wei Chen,
Wei Chen,
Yang Dong,
Yang Dong,
Yang Dong,
Yang Dong

Affiliations

Ying Liang: Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Shanshan Chen: BGI College, Zhengzhou University, Zhengzhou, China
Kunhua Wei: Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Zijiang Yang: National and Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, China
Shengchang Duan: NowBio Biotechnology Company, Kunming, China
Yuan Du: NowBio Biotechnology Company, Kunming, China
Peng Qu: Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Jianhua Miao: Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Wei Chen: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, China
Wei Chen: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China
Wei Chen: Yunnan Research Institute for Local Plateau Agriculture and Industry, Kunming, China
Yang Dong: Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Yang Dong: National and Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, China
Yang Dong: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, China
Yang Dong: Yunnan Research Institute for Local Plateau Agriculture and Industry, Kunming, China

DOI: https://doi.org/10.3389/fgene.2020.00701
Journal volume & issue: Vol. 11

Abstract

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Andrographis paniculata (Chinese name: Chuanxinlian) is an annual dicotyledonous medicinal plant widely grown in China and Southeast Asia. The dried plant has a highly acclaimed usage in the traditional Chinese medicine for its antipyretic, anti-inflammatory, and analgesic effects. In order to help delineate the biosynthetic pathways of various secondary metabolites, we report in this study a high-quality reference genome for A. paniculata. With the help of both PacBio single molecule real time sequencing and Illumina sequencing reads for error correction, the A. paniculata genome was assembled into a total size of 284 Mb with a contig N50 size of 5.14 Mb. The contigs were further assembled into 24 pseudo-chromosomes by the Hi-C technique. We also analyzed the gene families (e.g., KSL, and CYP450) whose protein products are essential for synthesizing bioactive compounds in A. paniculata. In conclusion, the high-quality A. paniculata genome assembly builds the foundation for decoding the biosynthetic pathways of various medicinal compounds.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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