Evaluation and analysis of pine resin components of Pinus elliottii clones

Abstract

In order to select superior clones with strong resin-producing capacity and high resin-quality from the existing fast-growing timber clones in seed orchard of Pinus elliottii, and to make high use of crop germplasm resource, 36 clones in the first generation of slash pine seed orchard were used as materials to determine their resin yield, resin mass flow rate and DBH growth, and to analyze their resin composition by GC-MS. Based on the above indicators, correlation analysis and cluster analysis was used to comprehensively evaluate the production and quality of resin among 36 clones. The results were as follows: (1) There were a total of 21 pine resin components, including 8 monoterpenes and 13 diterpenes. (2) Correlation analysis showed that the resin mass flow rate (RMR) had significant and positive correlation with the total content of monoterpene, weakly negatively correlates to abietic-type resin acid, and not significantly correlated to pimaric-type resin acid. (3) Based on the cluster analysis results integrating four types of indicators as the total monoterpene content, resin mass flow rate, abietic-type resin acid and pimaric-type resin acid, 36 clones could be divided into three categories and the difference between each type was significant. The performance of Class 1 was much better than that of the other two categories. (4) There were 17 high-resin yield pine clones (ERM≥15.15) among 36 clones, and on the basis of this, four clones (6-44, 4-11-1, 1-38, 3-64) display higher monoterpenes content, while four clones (4-11-1, 3-64, 2-0420, 3-468) showed higher contents of pimaric-type resin acid. And the content of abietic-type resin acid of clone 2-173 was the highest. In summary, a total of 21 pine resin components of P. elliottii were identified, and 36 clones were evaluated based on four indicators: the total monoterpene content, resin mass flow rate, abietic-type resin acid and pimaric-type resin acid. We not only analyzed qualitatively the resin composition but also evaluated quantitatively the resin-producing capacity of 36 clones in slash pine seed orchard. Our findings provide the scientific references for the targeted breeding of pine resin components and lay a foundation for subsequent heredity breeding and gene improvement of P. elliottii.

Keywords

• pinus elliottii
• pine resin components
• correlation
• cluster analysis
• resin-producing capacity