Evaluation of the Expression Level of miR-32 and PTEN in Melanoma

Abstract

Background and purpose: The aim is to investigate the change in the expression level of miR-32 and PTEN genes in tumor samples with melanoma. Materials and methods: This study collected tumor samples from 50 patients and 50 samples from healthy marginal tissue around the tumor as a control sample. Following that, the RNA related to the samples was extracted and the expression of the desired gene was evaluated using real-time polymerase chain reaction. Appropriate statistical tests assessed differences in expression levels of the two groups. In statistical tests. Results: The expression of PTEN was lower in tumor samples than in healthy samples. In contrast, the expression of miR-32 was higher in tumor samples and the difference was significant in both cases (P<0.05). Conclusion: Our findings indicated that the miR-32 and PTEN genes can be used as diagnostic or predictive biomarkers in melanoma. However, it is recommended that further research be conducted in different communities with larger sample sizes. Similar tests can contribute to the immediate diagnosis of melanoma. Using the potential of these biomarkers for diagnosis, early detection of melanoma and better treatment will become attainable and more successful.

Keywords

• melanoma
• mir-32
• pten
• real time-pcr