Effects of Tramadol Administration on Sperm Characteristics on Testicular Ischemia-Reperfusion Injury in Rat

Abstract

Objective: The main purpose of this study was to instigate effect of tramadol administration on sperm characteristics on testicular ischemia-reperfusion (IR) injury in male Wistar rats. Materials and Methods: Eighteen Wistar rats were randomly allocated into 3 experimental groups. (A) The control group had no received any medications, abdominal cavity was opened but no IR-induced. The IR group (B): abdominal cavity was opened, IR-induced without pre-medication. (C) Animal orally administrated with tramadol (40 mg/kg) for 1 week followed by testicular IR-induced. The time duration of IR was 60 minutes in all experimental groups. Then after 24 hours, the abdomen was opened, semen samples were collected from the caudal epididymis to determine epididymis weight, sperm mortality, mobility and sperm count. Results: According to the results, sperm mortality, mobility and sperm count significantly diminished in control group rats (group B) (P < 0.05). Pre-treatment with 40 mg/kg of tramadol for 1 week before the IR was able to diminish effect of ischemia on sperm mortality, mobility and sperm count in rat (P < 0.05). Conclusion: Perhaps tramadol has curative effect against testicular IR.

Keywords

• Tramadol
• Sperm characteristic
• Ischemia-reperfusion
• Testicular
• Rats