Argonaute bypasses cellular obstacles without hindrance during target search

Tao Ju Cui; Misha Klein; Jorrit W. Hegge; Stanley D. Chandradoss; John van der Oost; Martin Depken; Chirlmin Joo

doi:10.1038/s41467-019-12415-y

Nature Communications (Sep 2019)

Argonaute bypasses cellular obstacles without hindrance during target search

Tao Ju Cui,
Misha Klein,
Jorrit W. Hegge,
Stanley D. Chandradoss,
John van der Oost,
Martin Depken,
Chirlmin Joo

Affiliations

Tao Ju Cui: Kavli Institute of Nanoscience and Department of Bionanoscience, Delft University of Technology
Misha Klein: Kavli Institute of Nanoscience and Department of Bionanoscience, Delft University of Technology
Jorrit W. Hegge: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University
Stanley D. Chandradoss: Kavli Institute of Nanoscience and Department of Bionanoscience, Delft University of Technology
John van der Oost: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University
Martin Depken: Kavli Institute of Nanoscience and Department of Bionanoscience, Delft University of Technology
Chirlmin Joo: Kavli Institute of Nanoscience and Department of Bionanoscience, Delft University of Technology

DOI: https://doi.org/10.1038/s41467-019-12415-y
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and slides along the DNA during target search.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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