Frontiers in Bioengineering and Biotechnology (Jun 2020)
Curcumin Protects Osteoblasts From Oxidative Stress-Induced Dysfunction via GSK3β-Nrf2 Signaling Pathway
- Xumin Li,
- Xumin Li,
- Xumin Li,
- Xumin Li,
- Yang Chen,
- Yang Chen,
- Yixin Mao,
- Yixin Mao,
- Yixin Mao,
- Panpan Dai,
- Xiaoyu Sun,
- Xiaoyu Sun,
- Xiaoyu Sun,
- Xiaorong Zhang,
- Xiaorong Zhang,
- Haoran Cheng,
- Haoran Cheng,
- Yingting Wang,
- Yingting Wang,
- Isaac Banda,
- Isaac Banda,
- Gang Wu,
- Jianfeng Ma,
- Jianfeng Ma,
- Shengbin Huang,
- Shengbin Huang,
- Shengbin Huang,
- Tim Forouzanfar
Affiliations
- Xumin Li
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Xumin Li
- Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam, MOVE Research Institute, University of Amsterdam and Vrije University Amsterdam, Amsterdam, Netherlands
- Xumin Li
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Xumin Li
- Department of Oral and Maxillofacial Surgary/Pathology, Amsterdam UMC and Academic Centre for Dentistry Amsterdam, Amsterdam Movement Science, Vrije Universitetit Amsterdam, Amsterdam, Netherlands
- Yang Chen
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yang Chen
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yixin Mao
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yixin Mao
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yixin Mao
- Laboratory for Myology, Amsterdam Movement Sciences, Faculty of Behavioral and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, Netherlands
- Panpan Dai
- Department of Stomatology, Taizhou Hospital, Wenzhou Medical University, Linhai, China
- Xiaoyu Sun
- Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam, MOVE Research Institute, University of Amsterdam and Vrije University Amsterdam, Amsterdam, Netherlands
- Xiaoyu Sun
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Xiaoyu Sun
- Department of Periodontology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Xiaorong Zhang
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Xiaorong Zhang
- Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Haoran Cheng
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Haoran Cheng
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yingting Wang
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yingting Wang
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Isaac Banda
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Isaac Banda
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Gang Wu
- Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam, MOVE Research Institute, University of Amsterdam and Vrije University Amsterdam, Amsterdam, Netherlands
- Jianfeng Ma
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Jianfeng Ma
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Shengbin Huang
- Department of Prosthodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Shengbin Huang
- Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam, MOVE Research Institute, University of Amsterdam and Vrije University Amsterdam, Amsterdam, Netherlands
- Shengbin Huang
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Tim Forouzanfar
- Department of Oral and Maxillofacial Surgary/Pathology, Amsterdam UMC and Academic Centre for Dentistry Amsterdam, Amsterdam Movement Science, Vrije Universitetit Amsterdam, Amsterdam, Netherlands
- DOI
- https://doi.org/10.3389/fbioe.2020.00625
- Journal volume & issue
-
Vol. 8
Abstract
Osteoblasts dysfunction, induced by oxidative stress (OS), is one of major pathological mechanisms for osteoporosis. Curcumin (Cur), a bioactive antioxidant compound, isolated from Curcumin longa L, was regarded as a strong reactive oxygen species (ROS) scavenger. However, it remains unveiled whether Cur can prevent osteoblasts from OS-induced dysfunction. To approach this question, we adopted a well-established OS model to investigate the preventive effect of Cur on osteoblasts dysfunction by measuring intracellular ROS production, cell viability, apoptosis rate and osteoblastogenesis markers. We showed that the pretreatment of Cur could significantly antagonize OS so as to suppress endogenous ROS production, maintain osteoblasts viability and promote osteoblastogenesis. Inhibiting Glycogen synthase kinase (GSK3β) and activating nuclear factor erythroid 2 related factor 2 (Nrf2) could significantly antagonize the destructive effects of OS, which indicated the critical role of GSK3β-Nrf2 signaling. Furthermore, Cur also abolished the suppressive effects of OS on GSK3β-Nrf2 signaling pathway. Our findings demonstrated that Cur could protect osteoblasts against OS-induced dysfunction via GSK3β-Nrf2 signaling and provide a promising way for osteoporosis treatment.
Keywords
