Simultaneous Determination of Three Effective Components in Different Compatibility Proportion of the Couple of Sophora flavescens-Fructus Ligustri lucidi by HPLC

Abstract

Sophora flavescens - Ligustrum lucidum has the effect of increasing the anti-cancer effect of white blood cells and treating liver fibrosis. In order to determine the optimal compatibility ratio of this drug pair, the rationality of the drug pairs in different proportions was revealed. In this experiment, three active ingredients of the Sophora flavescens-Fructus Ligustri lucidi were extracted by reflux extraction, which were matrine, oxymatrine and specnuezhenide. A method for simultaneously determining the content of matrine, oxymatrine and specnuezhenide in a short time by HPLC was established. The method is simple, precise, and stable. Reproducibility can be used to determine the content of three active ingredients of matrine, oxymatrineand specnuezhenide. The analysis results was indicated that the different proportions of compatibility and different extraction solvents have significant effects on the content of active ingredients and dissolution rate of Sophora flavescens- Fructus Ligustri lucidi, after the combination of Sophora flavescens- Fructus Ligustri lucidi in different proportions, the content of matrine, oxymatrine and specnuezhenide is greater than the content of the active ingredients of each single herb. When the ratio of compatibility of Sophora flavescens-Ligustrum Ligustri lucidi is <1:1, it is beneficial to the dissolution of the three active ingredients. By determining the optimal compatibility ratio of this drug pair, provided a theoretical basis for the study of late pharmacodynamics and provide theoretical basis for clinicalapplication of traditional Chinese medicine.