Drug Design, Development and Therapy (Sep 2024)

Protective Effect of Fasudil on Testicular Ischemia-Reperfusion Injury in Rats

  • Kaya C,
  • Kapisiz A,
  • Eryilmaz S,
  • Karabulut R,
  • Turkyilmaz Z,
  • Inan MA,
  • Aydin GY,
  • Sonmez K

Journal volume & issue
Vol. Volume 18
pp. 4319 – 4326

Abstract

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Cem Kaya,1 Alparslan Kapisiz,1 Sibel Eryilmaz,1 Ramazan Karabulut,1 Zafer Turkyilmaz,1 Mehmet Arda Inan,2 Gizem Yaz Aydin,3 Kaan Sonmez1 1Departments of Pediatric Surgery, Gazi University Faculty of Medicine, Yenimahalle, Ankara, Turkey; 2Pathology, Gazi University Faculty of Medicine, Yenimahalle, Ankara, Turkey; 3Biochemistry, Gazi University Faculty of Medicine, Yenimahalle, Ankara, TurkeyCorrespondence: Ramazan Karabulut, Department of Pediatric Surgery, Gazi University, Faculty of Medicine, Emniyet Mahallesi, Mevlana Bulvarı, No. 29, Yenimahalle, Ankara, 06500, Turkey, Tel +90 312 2026210, Fax +90 312 2230528, Email [email protected]; [email protected]: Ischemia-reperfusion (I/R) injury to the testis can lead to organ damage, infertility, and subfertility. The goal of this study was to investigate the effects of fasudil on this devastating condition.Methods: Thirty male Long-Evans rats were divided into five groups: a control group (no torsion), rats administered fasudil (30 mg/kg, no torsion), rats subject to ischemia with no treatment (I) (I/R injury), injured rats that received treatment 1 (T1) (I/R with 30 mg/kg fasudil before detorsion), and injured rats that received treatment 2 (T2) (I/R with 30 mg/kg fasudil after detorsion). Serum levels of TNF-ɑ and IL-6, along with tissue levels of glutathione (GSH), malondialdehyde (MDA), caspase-3, and Johnsen Tubular Biopsy Score (JTBS), were measured.Results: Group I exhibited significantly higher levels of MDA and caspase-3 than all other groups except T2 (p ˂ 0.05). Although the difference was not statistically significant, Group T2 exhibited lower MDA and caspase-3 levels than Group I (p ˃ 0.05). Additionally, Group I displayed significantly higher TNF-ɑ and IL-6 levels, and lower GSH and JTBS values, than the other groups (p ˂ 0.05).Conclusion: Our findings indicate that fasudil protects the testis from I/R injury, particularly when administered early.Keywords: fasudil, testis, ischemia/reperfusion

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