MicroRNAs-1299, -126-3p and -30e-3p as Potential Diagnostic Biomarkers for Prediabetes

Cecil J. Weale; Don M. Matshazi; Saarah F. G. Davids; Shanel Raghubeer; Rajiv T. Erasmus; Andre P. Kengne; Glenda M. Davison; Tandi E. Matsha

doi:10.3390/diagnostics11060949

Diagnostics (May 2021)

MicroRNAs-1299, -126-3p and -30e-3p as Potential Diagnostic Biomarkers for Prediabetes

Cecil J. Weale,
Don M. Matshazi,
Saarah F. G. Davids,
Shanel Raghubeer,
Rajiv T. Erasmus,
Andre P. Kengne,
Glenda M. Davison,
Tandi E. Matsha

Affiliations

Cecil J. Weale: SAMRC/CPUT/ Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7530, South Africa
Don M. Matshazi: SAMRC/CPUT/ Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7530, South Africa
Saarah F. G. Davids: SAMRC/CPUT/ Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7530, South Africa
Shanel Raghubeer: SAMRC/CPUT/ Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7530, South Africa
Rajiv T. Erasmus: Division of Chemical Pathology, Faculty of Health Sciences, National Health Laboratory Service (NHLS) and University of Stellenbosch, Cape Town 8000, South Africa
Andre P. Kengne: Non-Communicable Diseases Research Unit, South African Medical Research Council, Cape Town 7505, South Africa
Glenda M. Davison: SAMRC/CPUT/ Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7530, South Africa
Tandi E. Matsha: SAMRC/CPUT/ Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7530, South Africa

DOI: https://doi.org/10.3390/diagnostics11060949
Journal volume & issue: Vol. 11, no. 6
p. 949

Abstract

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This cross-sectional study investigated the association of miR-1299, -126-3p and -30e-3p with and their diagnostic capability for dysglycaemia in 1273 (men, n = 345) South Africans, aged >20 years. Glycaemic status was assessed by oral glucose tolerance test (OGTT). Whole blood microRNA (miRNA) expressions were assessed using TaqMan-based reverse transcription quantitative-PCR (RT-qPCR). Receiver operating characteristic (ROC) curves assessed the ability of each miRNA to discriminate dysglycaemia, while multivariable logistic regression analyses linked expression with dysglycaemia. In all, 207 (16.2%) and 94 (7.4%) participants had prediabetes and type 2 diabetes mellitus (T2DM), respectively. All three miRNAs were significantly highly expressed in individuals with prediabetes compared to normotolerant patients, p p p = 0.042. These results suggest that miR-1299, -126-3p and -30e-3p are associated with prediabetes, and measuring miR-126-3p could potentially contribute to diabetes risk screening strategies.

Published in Diagnostics

ISSN: 2075-4418 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/diagnostics

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