Nature Communications (Jul 2022)
Partial freezing of rat livers extends preservation time by 5-fold
- Shannon N. Tessier,
- Reinier J. de Vries,
- Casie A. Pendexter,
- Stephanie E. J. Cronin,
- Sinan Ozer,
- Ehab O. A. Hafiz,
- Siavash Raigani,
- Joao Paulo Oliveira-Costa,
- Benjamin T. Wilks,
- Manuela Lopera Higuita,
- Thomas M. van Gulik,
- Osman Berk Usta,
- Shannon L. Stott,
- Heidi Yeh,
- Martin L. Yarmush,
- Korkut Uygun,
- Mehmet Toner
Affiliations
- Shannon N. Tessier
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Reinier J. de Vries
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Casie A. Pendexter
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Stephanie E. J. Cronin
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Sinan Ozer
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Ehab O. A. Hafiz
- Department of Electron Microscopy Research, Theodor Bilharz Research Institute
- Siavash Raigani
- Shriners Hospitals for Children Boston
- Joao Paulo Oliveira-Costa
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Benjamin T. Wilks
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Manuela Lopera Higuita
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Thomas M. van Gulik
- Department of Surgery, Amsterdam University Medical Centers – location AMC, University of Amsterdam
- Osman Berk Usta
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Shannon L. Stott
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Heidi Yeh
- Department of Surgery, Division of Transplantation, Massachusetts General Hospital
- Martin L. Yarmush
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Korkut Uygun
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- Mehmet Toner
- Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital
- DOI
- https://doi.org/10.1038/s41467-022-31490-2
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 13
Abstract
The limited preservation duration of donor organs is a big problem. Here the authors report a method for the freezing of whole rat livers at temperatures between −10 °C to −15 °C for up to 5 days, based on freeze-tolerant wood frogs, and term this partial freezing.
