Frontiers in Microbiology (Sep 2024)
Outer membrane vesicles secreted from Actinobacillus pleuropneumoniae isolate disseminating the floR resistance gene to Enterobacteriaceae
- Minsheng Xu,
- Minsheng Xu,
- Minsheng Xu,
- Minsheng Xu,
- Haiyi Ke,
- Yingan Zang,
- Hongchao Gou,
- Hongchao Gou,
- Hongchao Gou,
- Hongchao Gou,
- Dongxia Yang,
- Dongxia Yang,
- Dongxia Yang,
- Dongxia Yang,
- Keda Shi,
- Keda Shi,
- Keda Shi,
- Keda Shi,
- Kunli Zhang,
- Kunli Zhang,
- Kunli Zhang,
- Kunli Zhang,
- Yan Li,
- Yan Li,
- Yan Li,
- Yan Li,
- Zhiyong Jiang,
- Zhiyong Jiang,
- Zhiyong Jiang,
- Zhiyong Jiang,
- Pinpin Chu,
- Pinpin Chu,
- Pinpin Chu,
- Pinpin Chu,
- Shaolun Zhai,
- Shaolun Zhai,
- Shaolun Zhai,
- Shaolun Zhai,
- Chunling Li,
- Chunling Li,
- Chunling Li,
- Chunling Li
Affiliations
- Minsheng Xu
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Minsheng Xu
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Minsheng Xu
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Minsheng Xu
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Haiyi Ke
- Guangdong Gaozhou Agricultural School, Maoming, China
- Yingan Zang
- College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China
- Hongchao Gou
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Hongchao Gou
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Hongchao Gou
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Hongchao Gou
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Dongxia Yang
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Dongxia Yang
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Dongxia Yang
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Dongxia Yang
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Keda Shi
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Keda Shi
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Keda Shi
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Keda Shi
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Kunli Zhang
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Kunli Zhang
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Kunli Zhang
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Kunli Zhang
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Yan Li
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Yan Li
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Yan Li
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Yan Li
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Zhiyong Jiang
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Zhiyong Jiang
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Zhiyong Jiang
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Zhiyong Jiang
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Pinpin Chu
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Pinpin Chu
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Pinpin Chu
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Pinpin Chu
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Shaolun Zhai
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Shaolun Zhai
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Shaolun Zhai
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Shaolun Zhai
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- Chunling Li
- Guangdong Academy of Agricultural Sciences, Institute of Animal Health, Guangzhou, China
- Chunling Li
- Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangzhou, China
- Chunling Li
- Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, China
- Chunling Li
- Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Guangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2024.1467847
- Journal volume & issue
-
Vol. 15
Abstract
Actinobacillus pleuropneumoniae, a significant respiratory pig pathogen, is causing substantial losses in the global swine industry. The resistance spectrum of A. pleuropneumoniae is expanding, and multidrug resistance is a severe issue. Horizontal gene transfer (HGT) plays a crucial role in the development of the bacterial genome by facilitating the dissemination of resistance determinants. However, the horizontal transfer of resistance genes via A. pleuropneumoniae-derived outer membrane vesicles (OMVs) has not been previously reported. In this study, we used Illumina NovaSeq and PacBio SequeI sequencing platforms to determine the whole genome sequence of A. pleuropneumoniae GD2107, a multidrug-resistant (MDR) isolate from China. We detected a plasmid in the isolate named pGD2107-1; the plasmid was 5,027 bp in size with 7 putative open reading frames (ORF) and included the floR resistance genes. The carriage of resistance genes in A. pleuropneumoniae OMVs was identified using a polymerase chain reaction (PCR) assay, and then we thoroughly evaluated the influence of OMVs on the horizontal transfer of drug-resistant plasmids. The transfer of the plasmid to recipient bacteria via OMVs was confirmed by PCR. In growth competition experiments, all recipients carrying the pGD2107-1 plasmid exhibited a fitness cost compared to the corresponding original recipients. This study revealed that OMVs could mediate interspecific horizontal transfer of the resistance plasmid pGD2107-1 into Escherichia coli recipient strains and significantly enhance the resistance of the transformants. In summary, A. pleuropneumoniae-OMVs play the pivotal role of vectors for dissemination of the floR gene spread and may contribute to more antimicrobial resistance gene transfer in other Enterobacteriaceae.
Keywords
- Actinobacillus pleuropneumoniae
- whole genome shotgun
- horizontal gene transfer
- resistance plasmid
- outer membrane vesicles (OMVs)
