Effect of Carboxyl-Terminal Truncation on the Catalytic Performance of D-Phenylglycine Aminotransferase

Abstract

The D-phenylglycine aminotransferase (D-PhgAT) is a novel enzyme that can be used to synthesize precursors of antibiotics. This research addressed the function of the carboxyl-terminal (C-terminal) of DPhgAT. Its C-terminal amino acid sequence was compared to other related proteins using bioinformatics tools. The analyzed amino acid sequence was used to produce a genetically modified enzyme having a truncation of the 10 amino acid residues at the C-terminal region. The truncated D-PhgAT was purified and analyzed for catalytic performance. The results revealed that the truncated enzyme had better catalytic performance than the full-length enzyme by 37.49%. This research is a preliminary study for improving the enzymatic performance of D-PhgAT by structure-guided engineering and can be applied in the development of other enzymes.

Keywords

• D-phenylglycine aminotransferase
• Carboxyl-terminus
• Catalytic performance