Reproductive Biology and Endocrinology (Jan 2008)

Differential regulation of insulin-like growth factor binding protein-1 and -2 by insulin in the baboon (<it>Papio anubis</it>) endometrium

  • Fazleabas Asgerally T,
  • Fleming Steven D,
  • Bell Stephen C

DOI
https://doi.org/10.1186/1477-7827-6-6
Journal volume & issue
Vol. 6, no. 1
p. 6

Abstract

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Abstract Background The purpose of this study was to examine the effect of insulin on expression and synthesis of IGFBP-1 and IGFBP-2 in the baboon endometrium in vitro. Methods Baboon endometrial explants collected from cycling, ovariectomized, steroid-treated, simulated-pregnant and pregnant animals were cultured for 48 h in the presence or absence of insulin, with or without estradiol, progesterone and hCG. Results Insulin clearly inhibited IGFBP-1 production and mRNA expression in a time- and dose-dependent manner, whereas IGFBP-2 synthesis was not significantly affected. The inhibitory effects of insulin on IGFBP-1 were more evident in explants of non-pregnant tissue or tissue away from the implantation site. In the absence of insulin, synthesis of IGFBP-1 was induced in explants with low levels of de novo synthesis whereas IGFBP-2 synthesis was inhibited. This effect was potentiated by steroids and hCG in the explant cultures. Conclusion Insulin differentially regulates endometrial IGFBP-1 and IGFBP-2 secretion in the baboon.