eLife (Jun 2019)

The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity

  • Mohammed Jamshad,
  • Timothy J Knowles,
  • Scott A White,
  • Douglas G Ward,
  • Fiyaz Mohammed,
  • Kazi Fahmida Rahman,
  • Max Wynne,
  • Gareth W Hughes,
  • Günter Kramer,
  • Bernd Bukau,
  • Damon Huber

DOI
https://doi.org/10.7554/eLife.48385
Journal volume & issue
Vol. 8

Abstract

Read online

In bacteria, the translocation of proteins across the cytoplasmic membrane by the Sec machinery requires the ATPase SecA. SecA binds ribosomes and recognises nascent substrate proteins, but the molecular mechanism of nascent substrate recognition is unknown. We investigated the role of the C-terminal tail (CTT) of SecA in nascent polypeptide recognition. The CTT consists of a flexible linker (FLD) and a small metal-binding domain (MBD). Phylogenetic analysis and ribosome binding experiments indicated that the MBD interacts with 70S ribosomes. Disruption of the MBD only or the entire CTT had opposing effects on ribosome binding, substrate-protein binding, ATPase activity and in vivo function, suggesting that the CTT influences the conformation of SecA. Site-specific crosslinking indicated that F399 in SecA contacts ribosomal protein uL29, and binding to nascent chains disrupts this interaction. Structural studies provided insight into the CTT-mediated conformational changes in SecA. Our results suggest a mechanism for nascent substrate protein recognition.

Keywords