Biosorption of Malachite Green by Dry Cells of Isolated Free Living Nitrogen Fixing Bacteria

Abstract

Contamination of water with Malachite green (MG) may threaten aquatic and human life. Nitrogen-fixing Azotobacter sp. is an efficient adsorbent for the removal of MG from dye solutions. The optimum pH for the biosorption process was determined. The maximum adsorption capacity and the effect of different adsorbate concentrations were detected. The kinetics and isotherm models for biosorption were constructed. Optimum adsorption of MG by Azotobacter sp. was obtained at pH 6.0, biomass concentration was 0.05%, initial dye concentration was 50 mg.L-1, and contact time was 600 mins. Dye adsorption exhibited an increase with contact time and initial malachite green concentration. The kinetics of the adsorption process was best followed by the pseudo-second-order kinetic model which confirms the chemisorption process. The adsorption equilibrium data fit well to the Langmuir model indicating a monolayer adsorption behavior onto a surface of Azotobacter sp. with a finite number of active sites. Maximum biosorption capacity was found to be 142.8 mg.g-1 of bacterial biomass. The dry biomass of Azotobacter sp. has proved to be an efficient biosorbent for the removal of synthetic dyes from actual industrial effluent that is contaminated with up to 400 mg.L-1 dye concentration.

Keywords

• azotobacter, biosorption, malachite green, nitrogen fixing bacteria