PROPAGATION OF A RARE ORNAMENTAL CLIMBER PLANT PORANA PANICULATA BY TISSUE CULTURE

Abstract

This work was carried out in the Tissue Culture Laboratory, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt during the period from 2015 to 2017, to investigate some factors affecting propagation of the rare ornamental climber Porana paniculata by tissue culture. Results could be briefed in the following: using clorox at 20% as a sterilant achieved the highest position in survival % and the lowest one concerning contamination %. The highest position was also occupied by clorox at 5% in relation to contamination %. Exposing explants to clorox for either 15 or 20 min gave rise to the highest survival %. Explants exposed to clorox at 20% for 15 min obtained the highest position in survival %, the pre-lowest rank in contamination % and the lowest one regarding mortality %. Using BAP at 0.60 ppm gave rise to the highest position in lateral buds survival %, regeneration capacity, culture efficiency, shoot number, shoot length and leaf number. Using 1/2 MS media induced the 1st position concerning lateral buds survival %, regeneration capacity, culture efficiency, shoot number, shoot length and leaf number. Using BAP at 0.60 ppm + 1/2 MS medium resulted in the highest records of lateral buds survival %, regeneration capacity, culture efficiency, shoot number, shoot length and leaf number. BAP at 0.40 ppm + 1/2 MS medium gave the 1st rank of shoot number. Using BAP at 1 ppm induced the 1st position regarding shoot survival %, shoot number, shoot length and leaf number. NAA at 0.1 ppm resulted in the 1st position regarding shoot survival %, shoot number, shoot length and leaf number. BAP at 1 ppm + NAA at 0.1 ppm recorded the 1st position concerning shoot survival %, shoot number, shoot length and leaf number.

Keywords

• porana paniculata
• tissue culture
• clorox
• bap
• ms medium
• naa