The impact of mariculture biofilters on the distribution of benthic nutrient fluxes, organic matters and bacterial community in a mariculture wastewater treatment system

Abstract

Constructed-wetlands, biofilms, and sedimentation are among biological filters used in mariculture wastewater treatments, however, their impacts on the distribution of benthic microbial community and inorganic-nutrient fluxes have not been fully explored. This study applied 16 S rRNA high-throughput sequencing technology to investigate the microbial community distribution and their impacts on nutrient fluxes in mariculture biofilters system. Results showed that bacterial community compositions were significantly different in the constructed-wetland and biofilm treatments (p < 0.05) relative to sedimentation. The composition of the 16 S rRNA genes among the treatments were enriched with Proteobacteria (73%), Bacteroidetes (69%), Firmicutes (62%), and Flavobacteria (61%) in Biofilm compared to Proteobacteria (53%), Bacteroidetes (39%), Firmicutes (32%), and Flavobacteria (21%) in constructed wetlands. NMDS analysis showed that bacterial composition in constructed-wetland and biofilms clustered separately compared to sedimentation treatment. Functional-Annotation-of-Prokaryotic-Taxa analysis indicated that the proportions of sediment-microbial-functional groups (aerobic-chemoheterotrophy, chemoheterotrophy, and nitrate-ammonification) were 47% in the constructed-wetland, 32% in biofilm and 13% in sedimentation system. Benthic-nutrient fluxes for phosphate, ammonium, nitrite, nitrate and sediment oxygen consumption differed markedly among the treatments (p < 0.05). Canonical correspondence analysis indicated constructed-wetland had the strongest association between biogeochemical contents and the bacterial community relative to other treatments. This study suggests that the mariculture wastewater biofilters promoted microbial community distributions, sediment bacterial functional-groups including chemoheterotrophy, aerobic-chemoheterotrophy, denitrification, and nitrification and interactions with nutrient fluxes which was more pronounced in the constructed-wetland system.

Keywords

• Sediment bacteria community
• Wastewater
• Biofilm
• Constructed-wetland
• Mariculture
• High throughout sequencing