Aerosol Inhalation Delivery of Ceftriaxone in Mice: Generation Procedure, Pharmacokinetics, and Therapeutic Outcome

Sergey V. Valiulin; Andrei A. Onischuk; Anatoly M. Baklanov; Sergey V. An’kov; Sergey N. Dubtsov; Alexander A. Alekseev; Nikolay N. Shkil; Ekaterina V. Nefedova; Maria E. Plokhotnichenko; Tatyana G. Tolstikova; Arseniy M. Dolgov; Galina G. Dultseva

doi:10.3390/antibiotics11101305

Antibiotics (Sep 2022)

Aerosol Inhalation Delivery of Ceftriaxone in Mice: Generation Procedure, Pharmacokinetics, and Therapeutic Outcome

Sergey V. Valiulin,
Andrei A. Onischuk,
Anatoly M. Baklanov,
Sergey V. An’kov,
Sergey N. Dubtsov,
Alexander A. Alekseev,
Nikolay N. Shkil,
Ekaterina V. Nefedova,
Maria E. Plokhotnichenko,
Tatyana G. Tolstikova,
Arseniy M. Dolgov,
Galina G. Dultseva

Affiliations

Sergey V. Valiulin: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Andrei A. Onischuk: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Anatoly M. Baklanov: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Sergey V. An’kov: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Sergey N. Dubtsov: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Alexander A. Alekseev: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Nikolay N. Shkil: Siberian Federal Scientific Center of Agro-BioTechnologies RAS, 630501 Krasnoobsk, Russia
Ekaterina V. Nefedova: Siberian Federal Scientific Center of Agro-BioTechnologies RAS, 630501 Krasnoobsk, Russia
Maria E. Plokhotnichenko: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Tatyana G. Tolstikova: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Arseniy M. Dolgov: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia
Galina G. Dultseva: Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya Str., 630090 Novosibirsk, Russia

DOI: https://doi.org/10.3390/antibiotics11101305
Journal volume & issue: Vol. 11, no. 10
p. 1305

Abstract

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Aerosol inhalation delivery of ceftriaxone in mice was investigated. An ultrasonic nebulizer within the ranges of mean particle diameter 0.5–1.5 μm and mass concentration 0.01–0.6 μg/cm3 was used in inhalation experiments. Pharmacokinetic measurements were carried out using a nose-only chamber. Ceftriaxone concentration in blood serum and its mass in the lungs of mice were measured as a function of time using high-performance liquid chromatography. The body-delivered dose was within the range 3–5 mg/kg. The antibacterial effect of aerosolized ceftriaxone was investigated for mice infected with Klebsiella pneumoniae 82 and Staphylococcus aureus ATCC 25 953. The survival rate for infected mice after the treatment with ceftriaxone aerosol revealed the high antibacterial efficiency of this kind of treatment.

Published in Antibiotics

ISSN: 2079-6382 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antibiotics

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