Nature Communications (Dec 2017)
Robust RNA-based in situ mutation detection delineates colorectal cancer subclonal evolution
- Ann-Marie Baker,
- Weini Huang,
- Xiao-Ming Mindy Wang,
- Marnix Jansen,
- Xiao-Jun Ma,
- Jeffrey Kim,
- Courtney M. Anderson,
- Xingyong Wu,
- Liuliu Pan,
- Nan Su,
- Yuling Luo,
- Enric Domingo,
- Timon Heide,
- Andrea Sottoriva,
- Annabelle Lewis,
- Andrew D. Beggs,
- Nicholas A. Wright,
- Manuel Rodriguez-Justo,
- Emily Park,
- Ian Tomlinson,
- Trevor A. Graham
Affiliations
- Ann-Marie Baker
- Barts Cancer Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London
- Weini Huang
- Barts Cancer Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London
- Xiao-Ming Mindy Wang
- Advanced Cell Diagnostics
- Marnix Jansen
- Department of Histopathology, University College London Hospital
- Xiao-Jun Ma
- Advanced Cell Diagnostics
- Jeffrey Kim
- Advanced Cell Diagnostics
- Courtney M. Anderson
- Advanced Cell Diagnostics
- Xingyong Wu
- Advanced Cell Diagnostics
- Liuliu Pan
- Advanced Cell Diagnostics
- Nan Su
- Advanced Cell Diagnostics
- Yuling Luo
- Advanced Cell Diagnostics
- Enric Domingo
- Department of Oncology, Old Road Campus Research Building, University of Oxford
- Timon Heide
- Centre for Evolution and Cancer, The Institute of Cancer Research
- Andrea Sottoriva
- Centre for Evolution and Cancer, The Institute of Cancer Research
- Annabelle Lewis
- Cancer Gene Regulation Laboratory, Centre for Cancer Gene Research, Wellcome Trust Centre for Human Genetics, University of Oxford
- Andrew D. Beggs
- Surgical Research Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham
- Nicholas A. Wright
- Barts Cancer Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London
- Manuel Rodriguez-Justo
- Department of Histopathology, University College London Hospital
- Emily Park
- Advanced Cell Diagnostics
- Ian Tomlinson
- Cancer Genetics and Evolution Laboratory, Institute of Cancer and Genomic Sciences, University of Birmingham
- Trevor A. Graham
- Barts Cancer Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London
- DOI
- https://doi.org/10.1038/s41467-017-02295-5
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 8
Abstract
Methods that analyze intra-tumor genetic heterogeneity often do not preserve the spatial context of tumor subclones. Here, the authors present BaseScope, a mutation-specific RNA in situ hybridization assay and spatially map colorectal cancer and adenoma KRAS, BRAF and PIK3CA driver gene mutant subclones.
