Nature Communications (Aug 2021)
Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM
- Aleksandar Antanasijevic,
- Leigh M. Sewall,
- Christopher A. Cottrell,
- Diane G. Carnathan,
- Luis E. Jimenez,
- Julia T. Ngo,
- Jennifer B. Silverman,
- Bettina Groschel,
- Erik Georgeson,
- Jinal Bhiman,
- Raiza Bastidas,
- Celia LaBranche,
- Joel D. Allen,
- Jeffrey Copps,
- Hailee R. Perrett,
- Kimmo Rantalainen,
- Fabien Cannac,
- Yuhe R. Yang,
- Alba Torrents de la Peña,
- Rebeca Froes Rocha,
- Zachary T. Berndsen,
- David Baker,
- Neil P. King,
- Rogier W. Sanders,
- John P. Moore,
- Shane Crotty,
- Max Crispin,
- David C. Montefiori,
- Dennis R. Burton,
- William R. Schief,
- Guido Silvestri,
- Andrew B. Ward
Affiliations
- Aleksandar Antanasijevic
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Leigh M. Sewall
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Christopher A. Cottrell
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Diane G. Carnathan
- Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University
- Luis E. Jimenez
- Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University
- Julia T. Ngo
- Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University
- Jennifer B. Silverman
- Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University
- Bettina Groschel
- Department of Immunology and Microbiology, The Scripps Research Institute
- Erik Georgeson
- Department of Immunology and Microbiology, The Scripps Research Institute
- Jinal Bhiman
- Department of Immunology and Microbiology, The Scripps Research Institute
- Raiza Bastidas
- Department of Immunology and Microbiology, The Scripps Research Institute
- Celia LaBranche
- Department of Surgery, Duke University Medical Center
- Joel D. Allen
- School of Biological Sciences, University of Southampton
- Jeffrey Copps
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Hailee R. Perrett
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Kimmo Rantalainen
- Department of Immunology and Microbiology, The Scripps Research Institute
- Fabien Cannac
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Yuhe R. Yang
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Alba Torrents de la Peña
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Rebeca Froes Rocha
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- Zachary T. Berndsen
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- David Baker
- Institute for Protein Design, Department of Biochemistry, University of Washington
- Neil P. King
- Institute for Protein Design, Department of Biochemistry, University of Washington
- Rogier W. Sanders
- Academic Medical Center (AMC), University of Amsterdam
- John P. Moore
- Weill Cornell Medicine, Cornell University
- Shane Crotty
- La Jolla Institute for Immunology
- Max Crispin
- School of Biological Sciences, University of Southampton
- David C. Montefiori
- Department of Surgery, Duke University Medical Center
- Dennis R. Burton
- International AIDS Vaccine Initiative Neutralizing Antibody Center, the Collaboration for AIDS Vaccine Discovery (CAVD) and Scripps Consortium for HIV/AIDS Vaccine Development (CHAVD), The Scripps Research Institute
- William R. Schief
- International AIDS Vaccine Initiative Neutralizing Antibody Center, the Collaboration for AIDS Vaccine Discovery (CAVD) and Scripps Consortium for HIV/AIDS Vaccine Development (CHAVD), The Scripps Research Institute
- Guido Silvestri
- Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University
- Andrew B. Ward
- Department of Integrative, Structural and Computational Biology, The Scripps Research Institute
- DOI
- https://doi.org/10.1038/s41467-021-25087-4
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 17
Abstract
Here, the authors present cryoEMPEM, a method for high-resolution structural analysis of vaccine-elicited polyclonal antibody responses. They apply cryoEMPEM in combination with standard serology experiments to characterize the polyclonal antibody (pAb) responses elicited in rhesus macaques by HIV Env trimer immunogens and were able to determine up to 8 different polyclonal antibody structures in complex with their respective antigen from a single cryoEM dataset.
