ASSOCIATION BETWEEN POLYMORPHIC GENES OF FOLATE METABOLISM AND EARLY PREGNANCY LOSSES

Abstract

Aim: to analyze the polymorphic genes of folate metabolism in women with history of non-developing pregnancy and in their embryos/fetuses in order to relate the genetic polymorphism with the risk of early pregnancy loss.Materials and methods. The main group consisted of 117 women with non-developing 1st trimester pregnancy; the comparison group included 117 women with no history of miscarriage, with physiological 1st trimester pregnancy and a normal fetal karyotype. By polymerase chain reaction in real time using DT-96 machine («DNA-technology», Russia) all women and embryos were analyzed for the following polymorphic variants: MTHFR 677 C>T, MTHFR 1298 A>C, MTRR 66 A>G, MTR 2756 A>G.Results. The women with non-developing pregnancy and chromosomal abnormalities of the fetus had a significantly higher occurrence of the MTR 2756G allele in comparison with women with non-developing pregnancy and a normal karyotype of the fetus.Conclusion. The 2756G allele of the polymorphic MTR 2756 A>G gene in women with non-developing pregnancy is associated with a chromosomal imbalance of their fetuses.

Keywords

• non-developing pregnancy
• polymorphic genes of folate metabolism
• fetal karyotype