Evaluation of safety and antifungal activity of Lactobacillus reuteri and Pediococcus diacetilactis isolates against aflatoxigenic Aspergillus flavus

Abstract

Biocontrol of moulds by lactic acid bacteria (LAB) instead of antibiotics and chemical preservatives is a new approach in veterinary medicine. The aims of present research were to perform molecular identification of dominant sourdough LAB isolates and to evaluate their in vivo safety and in vitro antifungal properties for using as biopreservative agents. Sequencing results of PCR products led to identification of Lactobacillus reuteri and Pediococcus diacetilactis as LAB isolates. Antifungal ac-tivity of the isolates and their cell-free culture filtrate (CCF) were also confirmed against aflatoxigenic Aspergillus flavus, respectively by overlay and spore spot methods. Accordingly, antagonistic effect of P. diacetilactis and its CCF were significantly (P<0.05) higher than L. reuteri and CCF of mentioned LAB isolate. Clinical chemistry and haematological findings in mice fed LAB demonstrated also insignificant difference vs control mice and were in the normal range, which confirmed the safety of LAB isolates. By considering the importance of safe, food grade biocontrol agents, L. reuteri and P. diacetilactis isolates and their CCF may be considered as an alternative for antibiotics and other chemical preservatives in food and feed processing chain.

Keywords

• antifungal effect
• biocontrol
• lactic acid bacteria isolates
• safety