Communications Biology (Apr 2021)

Neutron reflectometry and NMR spectroscopy of full-length Bcl-2 protein reveal its membrane localization and conformation

  • Ameeq Ul Mushtaq,
  • Jörgen Ådén,
  • Luke A. Clifton,
  • Hanna Wacklin-Knecht,
  • Mario Campana,
  • Artur P. G. Dingeldein,
  • Cecilia Persson,
  • Tobias Sparrman,
  • Gerhard Gröbner

DOI
https://doi.org/10.1038/s42003-021-02032-1
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 10

Abstract

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Through neutron reflectometry and NMR spectroscopy studies, Mushtaq et al study the full-length Bcl-2 protein reconstituted in lipid bilayers. They find that, in contrast to previously studied truncated, soluble protein versions, intact Bcl-2 is mainly embedded in the membrane with its regulatory loop highly flexible.