Nature Communications (Mar 2021)
Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection
- Lukas Wettstein,
- Tatjana Weil,
- Carina Conzelmann,
- Janis A. Müller,
- Rüdiger Groß,
- Maximilian Hirschenberger,
- Alina Seidel,
- Susanne Klute,
- Fabian Zech,
- Caterina Prelli Bozzo,
- Nico Preising,
- Giorgio Fois,
- Robin Lochbaum,
- Philip Maximilian Knaff,
- Volker Mailänder,
- Ludger Ständker,
- Dietmar Rudolf Thal,
- Christian Schumann,
- Steffen Stenger,
- Alexander Kleger,
- Günter Lochnit,
- Benjamin Mayer,
- Yasser B. Ruiz-Blanco,
- Markus Hoffmann,
- Konstantin M. J. Sparrer,
- Stefan Pöhlmann,
- Elsa Sanchez-Garcia,
- Frank Kirchhoff,
- Manfred Frick,
- Jan Münch
Affiliations
- Lukas Wettstein
- Institute of Molecular Virology, Ulm University Medical Center
- Tatjana Weil
- Institute of Molecular Virology, Ulm University Medical Center
- Carina Conzelmann
- Institute of Molecular Virology, Ulm University Medical Center
- Janis A. Müller
- Institute of Molecular Virology, Ulm University Medical Center
- Rüdiger Groß
- Institute of Molecular Virology, Ulm University Medical Center
- Maximilian Hirschenberger
- Institute of Molecular Virology, Ulm University Medical Center
- Alina Seidel
- Institute of Molecular Virology, Ulm University Medical Center
- Susanne Klute
- Institute of Molecular Virology, Ulm University Medical Center
- Fabian Zech
- Institute of Molecular Virology, Ulm University Medical Center
- Caterina Prelli Bozzo
- Institute of Molecular Virology, Ulm University Medical Center
- Nico Preising
- Core Facility Functional Peptidomics, Ulm University Medical Center
- Giorgio Fois
- Institute of General Physiology, Ulm University
- Robin Lochbaum
- Institute of General Physiology, Ulm University
- Philip Maximilian Knaff
- Dermatology Clinic, University Medicine Mainz
- Volker Mailänder
- Dermatology Clinic, University Medicine Mainz
- Ludger Ständker
- Core Facility Functional Peptidomics, Ulm University Medical Center
- Dietmar Rudolf Thal
- Laboratory of Neuropathology, Department of Imaging and Pathology, KU-Leuven and Department of Pathology, UZ-Leuven
- Christian Schumann
- Pneumology, Thoracic Oncology, Sleep and Respiratory Critical Care Medicine, Clinics Allgäu
- Steffen Stenger
- Institute for Microbiology and Hygiene, Ulm University Medical Center
- Alexander Kleger
- Department of Internal Medicine 1, Ulm University Hospital
- Günter Lochnit
- Institute of Biochemistry, Justus-Liebig University Giessen
- Benjamin Mayer
- Institute for Epidemiology and Medical Biometry, Ulm University
- Yasser B. Ruiz-Blanco
- Computational Biochemistry, Center of Medical Biotechnology, University of Duisburg-Essen
- Markus Hoffmann
- Infection Biology Unit, German Primate Center- Leibniz institute for Primate Research
- Konstantin M. J. Sparrer
- Institute of Molecular Virology, Ulm University Medical Center
- Stefan Pöhlmann
- Infection Biology Unit, German Primate Center- Leibniz institute for Primate Research
- Elsa Sanchez-Garcia
- Computational Biochemistry, Center of Medical Biotechnology, University of Duisburg-Essen
- Frank Kirchhoff
- Institute of Molecular Virology, Ulm University Medical Center
- Manfred Frick
- Institute of General Physiology, Ulm University
- Jan Münch
- Institute of Molecular Virology, Ulm University Medical Center
- DOI
- https://doi.org/10.1038/s41467-021-21972-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 10
Abstract
Here, via screening of a polypeptide library from bronchoalveolar lavage, the authors identify and characterize α1-antitrypsin (α1AT) as SARS-CoV-2 inhibitor and show that α1AT binds and inactivates the serine protease TMPRSS2, which enzymatically primes the SARS-CoV-2 spike protein for membrane fusion.
