Association between TNFα - 308 G/A and IFN-γ +874A/T polymorphisms with oral lichen planus

Abstract

Background: Oral lichen planus (OLP) is a chronic inflammatory disease that affects 0.1% to 4% of the population. The disease causes lesions in the buccal mucosa, gingiva, and rarely in the palate. In this study, the relationship between OLP and TNFα-308 G / A and IFN-γ + 874A / T polymorphisms was investigated.Methods : In this case-control study, a sample of buccal mucosa was taken from 50 healthy individuals and 50 OLP patients referred to Kerman Faculty of Dentistry were included by easy sampling method. Then, the presence of TNFα-308 G / A and IFN-γ + 874A / T polymorphisms in the case and control groups was determined using ARM-PCR technique followed by sequencing.Results : Frequency of allele A and GA of TNF-α (-308G / A) gene was significantly higher than G and GG in OLP patients compared to control group (P-value <0.0001). Also, AA genotype of TNF-α (-308G / A) gene was not present in OLP patients. About IFN-γ + 874A / T polymorphism, the association between T allele and the risk of oral lichen planus was confirmed but it was not statistically significant (p-value: 0.068).Conclusion : There is a strong association between the A allele of TNF-α (-308G / A) and the risk of developing oral lichen planus, although this association between the IFN-γ + 874A / T genotype and Oral lichen planus was also confirmed, but it was not strong enough to be used to predict the likelihood of developing the disease.

Keywords

• oral lichen planus
• tnf-α gene
• ifn-γ gene
• polymorphism