Communications Biology (Jul 2022)

Genome-wide CRISPR screen for HSV-1 host factors reveals PAPSS1 contributes to heparan sulfate synthesis

  • Takeshi Suzuki,
  • Yoshitaka Sato,
  • Yusuke Okuno,
  • Fumi Goshima,
  • Tadahisa Mikami,
  • Miki Umeda,
  • Takayuki Murata,
  • Takahiro Watanabe,
  • Koichi Watashi,
  • Takaji Wakita,
  • Hiroshi Kitagawa,
  • Hiroshi Kimura

DOI
https://doi.org/10.1038/s42003-022-03581-9
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 11

Abstract

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A genome-wide CRISPR screen for HSV-1 host factors using near-haploid HAP1 cells revealed PAPSS1 as an essential factor for heparan sulfate biosynthesis and HSV-1 infection, and identified several other host factors also involved in both processes.