Biological responses of whitefish (Coregonus lavaretus L.) to reduced toxic impact: Metal accumulation, haematological, immunological, and histopathological alterations

Abstract

Bioaccumulation of the main pollutants in the organs of whitefish, as well as their haematological parameters, were examined dynamically over a 40-year period in historically contaminated Lake Imandra. A quantitative histological analysis was performed to assess the physiological state of whitefish and histopathologies of organs, as well as their physiological and biochemical functions in the current period of toxic load decline. Biological reactions of whitefish from the historically contaminated area have been greatly modified in contrast to those of whitefish from the never contaminated area of the lake, and this shift persisted even after approximately 20 years of toxic load decline. First, high antioxidant status supports the body's systems, smoothing over the negative consequences of metal toxicity, phagocytosis and inflammatory reactions. Moreover, the defence mechanism of whitefish from the historically contaminated area actively uses the oxidative systems of nonspecific immunity. Second, the adaptive strategy is aimed at improving gas exchange without compensatory proliferation of gill structure, which increases their functional surface and reduces the distance to the bloodstream, as well as increasing haemoglobin in maturing erythrocytes. Third, the higher efficiency of endo- and phagocytosis was confirmed by detecting increased monocytes and macrophages in the peripheral blood and decreased melano-macrophage centres in the fish kidney. Elevated accumulation of Fe, Cu, and Se may serve a sign of liver pathology, while elevated accumulation of Zn and Co already indicates kidney pathology, which is confirmed by histopathological alterations.

Keywords

• Water quality recovery
• Fish
• Metal accumulation
• Phagocytosis
• Endocytosis
• Histopathological lesions