Molecular Detection of AmpC Family Genes Encoding Antibiotic Resistance among Escherichia coli isolated from Patients with Urinary Tract Infection (UTI) in Najaf Hospitals

Abstract

One hundred and thirty significant bacteriuria were detected. The study showed higher incidence of UTI in females (73.08%) than males (26.9%). The E. coli isolates which grown on culture of all the 130 urine samples with significant bacteriauria were 60 ( 46.2 %) . The isolates which were resistant to ampicillin and amoxicillin 53(88.3%) . The ability of E. coli isolates to AmpC production were tested; the results have revealed that 23 (43.4%) isolates produce AmpC β-lactamase. PCR amplification results have shown that eighteen E. coli isolates possess AmpC β- lactamase gene. The results in this study showed 8 (44.4%) had blaFOX gene and 7(38.9%) had blaCIT while 5(27.8%) of E. coli isolates had blaDHA and 9(50%) had blaEBC gene.

Keywords

• Detection AmpC β-lactamaseEscherichia coli
• Prevalence blaEBCgene
• Urinary tract infection