Antibacterial Ingredients and Modes of the Methanol-Phase Extract from the Fruit of <i>Amomum villosum</i> Lour.

Abstract

Epidemics of infectious diseases threaten human health and society stability. Pharmacophagous plants are rich in bioactive compounds that constitute a safe drug library for antimicrobial agents. In this study, we have deciphered for the first time antibacterial ingredients and modes of the methanol-phase extract (MPE) from the fruit of Amomum villosum Lour. The results have revealed that the antibacterial rate of the MPE was 63.64%, targeting 22 species of common pathogenic bacteria. The MPE was further purified by high performance liquid chromatography (Prep-HPLC), and three different constituents (Fractions 1–3) were obtained. Of these, the Fraction 2 treatment significantly increased the cell membrane fluidity and permeability, reduced the cell surface hydrophobicity, and damaged the integrity of the cell structure, leading to the leakage of cellular macromolecules of Gram-positive and Gram-negative pathogens (p p A. villosum Lour., and should be useful for its application in the medicinal and food preservative industries against common pathogens.

Keywords

• <i>Amomum villosum</i> Lour.
• antibacterial compound
• antibacterial mechanism
• pathogen
• infectious disease
• pharmacophagous plant