Nature Communications (Apr 2020)
Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
- Tingting Jiang,
- Jordana M. Henderson,
- Kevin Coote,
- Yi Cheng,
- Hillary C. Valley,
- Xiao-Ou Zhang,
- Qin Wang,
- Luke H. Rhym,
- Yueying Cao,
- Gregory A. Newby,
- Hermann Bihler,
- Martin Mense,
- Zhiping Weng,
- Daniel G. Anderson,
- Anton P. McCaffrey,
- David R. Liu,
- Wen Xue
Affiliations
- Tingting Jiang
- RNA Therapeutics Institute, University of Massachusetts Medical School
- Jordana M. Henderson
- TriLink BioTechnologies
- Kevin Coote
- Cystic Fibrosis Foundation, CFFT Lab
- Yi Cheng
- Cystic Fibrosis Foundation, CFFT Lab
- Hillary C. Valley
- Cystic Fibrosis Foundation, CFFT Lab
- Xiao-Ou Zhang
- Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School
- Qin Wang
- School of Life Sciences and Technology, Tongji University
- Luke H. Rhym
- David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
- Yueying Cao
- RNA Therapeutics Institute, University of Massachusetts Medical School
- Gregory A. Newby
- Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT
- Hermann Bihler
- Cystic Fibrosis Foundation, CFFT Lab
- Martin Mense
- Cystic Fibrosis Foundation, CFFT Lab
- Zhiping Weng
- Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School
- Daniel G. Anderson
- David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
- Anton P. McCaffrey
- TriLink BioTechnologies
- David R. Liu
- Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT
- Wen Xue
- RNA Therapeutics Institute, University of Massachusetts Medical School
- DOI
- https://doi.org/10.1038/s41467-020-15892-8
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 9
Abstract
Cas9 base editors are promising tools for correcting pathogenic single nucleotide mutations. Here the authors chemically modify mRNA encoding the editor and the gRNA to enhance editing and broaden its application.
