A Preliminary Study on the Determination of Triploidy by Chromosome Analysis at the Different Stages of Development in Rainbow Trout, Oncorhynchus mykiss

Abstract

There are several methods for ploidy determination of fish, those have been developed by many researchers until to date. For these methods, the cost and the applicability of each stage of fish development are very important. The aim of this study was to compare the applicability of chromosome preparation in rainbow trout (Oncorhynchus mykiss) at the different stages of development, including the early and late stages of embryo, pre-larvae, post-larvae and fry stages. Chromosome preparations were modified from previous studies and successfully used in order to determine the ploidy in O. mykiss. At the end of applications, the best result for the applicability of chromosome preparation was obtained from the embryos at late stage. Also, it was determined that the chromosome numbers in diploid and triploid O. mykiss were 2n=56 and 3x=84, respectively, that were selected from the well-spred metaphase chromosomes.

Keywords

• embryos of rainbow trout
• chromosome preparation
• development stages