Effects of culvert length and flow rates of raw water transport on biofilm development and water quality

Abstract

Long-distance water transfer projects are important for water allocation. To enhance our understanding of biofilm growth and changes in water quality during raw water transfer, raw water through a long-distance non-full culvert at flow rates of 1.4−2.0 m/s was studied. The results revealed that: (1) the biofilm total cell count (TCC) and heterotrophic plate count (HPC) were the highest at a flow rate of 1.5 m/s, which were 3.7 × 104 cells/cm2 and 1.1 × 103 CFU/cm2, respectively; (2) Proteobacteria had the highest relative abundance (RA) among all samples, and the RA in biofilm (78.85%) was higher than that in water (48%−59%); (3) when the pollutants and biofilm were partially shed, the total phosphorus (TP), permanganate index (CODMn), and dissolved organic carbon (DOC) increased by 0.011, 0.36, and 0.5 mg/L at most, respectively; and (4) dissolved oxygen (DO) was sufficient during non-full flow water transport and nitrification occurred. The highest removal rates of ammonium nitrogen (NH+4-N) and nitrous nitrogen (-N) reached 27.16% and 66.76%, respectively. At the flow rates of 2.0 m/s, the efficiency decreased to 10.47% and 41.25%, respectively, due to the shedding of biofilm. HIGHLIGHTS Raw water flow over a long-distance non-full culvert studied using an experimental model.; Flow rate influenced biofilm biomass, and resulted in different bacterial community structure in bulk water and biofilm.; Nitrification in the long-distance non-full culvert was only 0.54%–1.2% that in the simulation experiment.;

Keywords

• biofilm
• microbial community
• nitrification
• non-full flow culvert
• water transfer project