Molecular Therapy: Nucleic Acids (Dec 2021)
11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography
- Anastasia V. Ozerskaya,
- Tatiana N. Zamay,
- Olga S. Kolovskaya,
- Nikolay A. Tokarev,
- Kirill V. Belugin,
- Natalia G. Chanchikova,
- Oleg N. Badmaev,
- Galina S. Zamay,
- Irina A. Shchugoreva,
- Roman V. Moryachkov,
- Vladimir N. Zabluda,
- Vladimir A. Khorzhevskii,
- Nikolay Shepelevich,
- Stanislav V. Gappoev,
- Elena A. Karlova,
- Anastasia S. Saveleva,
- Alexander A. Volzhentsev,
- Anna N. Blagodatova,
- Kirill A. Lukyanenko,
- Dmitry V. Veprintsev,
- Tatyana E. Smolyarova,
- Felix N. Tomilin,
- Sergey S. Zamay,
- Vladimir N. Silnikov,
- Maxim V. Berezovski,
- Anna S. Kichkailo
Affiliations
- Anastasia V. Ozerskaya
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Tatiana N. Zamay
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Olga S. Kolovskaya
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Nikolay A. Tokarev
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Kirill V. Belugin
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Natalia G. Chanchikova
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Oleg N. Badmaev
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Galina S. Zamay
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Irina A. Shchugoreva
- Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Roman V. Moryachkov
- Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia; Kirensky Institute of Physics, Krasnoyarsk, Russia
- Vladimir N. Zabluda
- Kirensky Institute of Physics, Krasnoyarsk, Russia
- Vladimir A. Khorzhevskii
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Krasnoyarsk Regional Pathology-Anatomic Bureau, Krasnoyarsk, Russia
- Nikolay Shepelevich
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Stanislav V. Gappoev
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Krasnoyarsk Regional Pathology-Anatomic Bureau, Krasnoyarsk, Russia
- Elena A. Karlova
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Anastasia S. Saveleva
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Alexander A. Volzhentsev
- Federal Siberian Research Clinical Centre Under the Federal Medical Biological Agency, Krasnoyarsk, Russia
- Anna N. Blagodatova
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia
- Kirill A. Lukyanenko
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Dmitry V. Veprintsev
- Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Tatyana E. Smolyarova
- Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia; Kirensky Institute of Physics, Krasnoyarsk, Russia
- Felix N. Tomilin
- Kirensky Institute of Physics, Krasnoyarsk, Russia
- Sergey S. Zamay
- Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia
- Vladimir N. Silnikov
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
- Maxim V. Berezovski
- Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Canada; Corresponding author: Maxim V. Berezovski, Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Canada.
- Anna S. Kichkailo
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Federal Research Center Krasnoyarsk Science- Center SB RAS, Krasnoyarsk, Russia; Corresponding author: Anna S. Kichkailo, Krasnoyarsk State Medical University Named After Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.
- Journal volume & issue
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Vol. 26
pp. 1159 – 1172
Abstract
Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized an 11C-labeled oligonucleotide primer and hybridized it to an anti-cancer DNA aptamer. The 11C-aptamer was applied for in vivo imaging of Ehrlich ascites carcinoma and its metastases in mice using PET/CT. The imaging experiments with the 11C-aptamer determined very small primary and secondary tumors of 3 mm2 and less. We also compared 11C imaging with the standard radiotracer, 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG), and found better selectivity of the 11C-aptamer to metastatic lesions in the metabolically active organs than 18F-FDG. 11C radionuclide with an ultra-short (20.38 min) half-life is considered safest for PET/CT imaging and does not cause false-positive results in heart imaging. Its combination with aptamers gives us high-specificity and high-contrast imaging of cancer cells and can be applied for PET/CT-guided drug delivery in cancer therapies.
