Frontiers in Cell and Developmental Biology (Jul 2021)
Human DC3 Antigen Presenting Dendritic Cells From Induced Pluripotent Stem Cells
- Taiki Satoh,
- Taiki Satoh,
- Taiki Satoh,
- Marcelo A. S. Toledo,
- Marcelo A. S. Toledo,
- Marcelo A. S. Toledo,
- Janik Boehnke,
- Janik Boehnke,
- Kathrin Olschok,
- Niclas Flosdorf,
- Niclas Flosdorf,
- Katrin Götz,
- Katrin Götz,
- Caroline Küstermann,
- Caroline Küstermann,
- Stephanie Sontag,
- Stephanie Sontag,
- Kristin Seré,
- Kristin Seré,
- Steffen Koschmieder,
- Tim H. Brümmendorf,
- Nicolas Chatain,
- Yoh-ichi Tagawa,
- Martin Zenke,
- Martin Zenke
Affiliations
- Taiki Satoh
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Taiki Satoh
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Taiki Satoh
- School of Life Sciences and Technology, Tokyo Institute of Technology, Kanagawa, Japan
- Marcelo A. S. Toledo
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Marcelo A. S. Toledo
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Marcelo A. S. Toledo
- Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Medical School, Aachen, Germany
- Janik Boehnke
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Janik Boehnke
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Kathrin Olschok
- Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Medical School, Aachen, Germany
- Niclas Flosdorf
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Niclas Flosdorf
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Katrin Götz
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Katrin Götz
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Caroline Küstermann
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Caroline Küstermann
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Stephanie Sontag
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Stephanie Sontag
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Kristin Seré
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Kristin Seré
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- Steffen Koschmieder
- Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Medical School, Aachen, Germany
- Tim H. Brümmendorf
- Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Medical School, Aachen, Germany
- Nicolas Chatain
- Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Medical School, Aachen, Germany
- Yoh-ichi Tagawa
- School of Life Sciences and Technology, Tokyo Institute of Technology, Kanagawa, Japan
- Martin Zenke
- Department of Cell Biology, Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany
- Martin Zenke
- Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany
- DOI
- https://doi.org/10.3389/fcell.2021.667304
- Journal volume & issue
-
Vol. 9
Abstract
Dendritic cells (DC) are professional antigen-presenting cells that develop from hematopoietic stem cells. Different DC subsets exist based on ontogeny, location and function, including the recently identified proinflammatory DC3 subset. DC3 have the prominent activity to polarize CD8+ T cells into CD8+ CD103+ tissue resident T cells. Here we describe human DC3 differentiated from induced pluripotent stem cells (iPS cells). iPS cell-derived DC3 have the gene expression and surface marker make-up of blood DC3 and polarize CD8+ T cells into CD8+ CD103+ tissue-resident memory T cells in vitro. To test the impact of malignant JAK2 V617F mutation on DC3, we differentiated patient-specific iPS cells with JAK2 V617Fhet and JAK2 V617Fhom mutations into JAK2 V617Fhet and JAK2 V617Fhom DC3. The JAK2 V617F mutation enhanced DC3 production and caused a bias toward erythrocytes and megakaryocytes. The patient-specific iPS cell-derived DC3 are expected to allow studying DC3 in human diseases and developing novel therapeutics.
Keywords
