Nature Communications (Dec 2021)
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids
- Alessandro Fiorenzano,
- Edoardo Sozzi,
- Marcella Birtele,
- Janko Kajtez,
- Jessica Giacomoni,
- Fredrik Nilsson,
- Andreas Bruzelius,
- Yogita Sharma,
- Yu Zhang,
- Bengt Mattsson,
- Jenny Emnéus,
- Daniella Rylander Ottosson,
- Petter Storm,
- Malin Parmar
Affiliations
- Alessandro Fiorenzano
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Edoardo Sozzi
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Marcella Birtele
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Janko Kajtez
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Jessica Giacomoni
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Fredrik Nilsson
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Andreas Bruzelius
- Regenerative Neurophysiology, Wallenberg Neuroscience Center, Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Yogita Sharma
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Yu Zhang
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Bengt Mattsson
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Jenny Emnéus
- Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark
- Daniella Rylander Ottosson
- Regenerative Neurophysiology, Wallenberg Neuroscience Center, Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Petter Storm
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- Malin Parmar
- Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, and Lund Stem Cell Center, Department of Experimental Medical Science, Lund University
- DOI
- https://doi.org/10.1038/s41467-021-27464-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 19
Abstract
3D brain organoids have been used to investigate human brain development and pathology. Here the authors establish human ventral midbrain organoids coupled with single cell sequencing to study developing and mature dopamine neurons and use silk scaffolding to generate bioengineered brain organoids
