Development diagnostic methods of bacterial blight of rice on based PCR

Abstract

Primers identifying bacterial blight pathogen, without cross-reactions with closely related species were designed based on the analysis of the original and GeneBank DNA sequences of Xanthomonas oryzae pv. oryzae. Media YPGA containing cycloheximide 50 mg / l + cefazolin 30 mg / l + gentamicin 2 mg / l has been optimized for BIO-PCR. Sensitivity of the BIO-PCR analysis using original selective media up to 100 times more effective than direct PCR analysis. The sensitivity of the primers in BIO-PCR analysis with original selective media has achieved 50 CFU/ml.

Keywords

• бактериальный ожог риса
• ПЦР «в реальном времени»
• БИО-ПЦР