International Journal of Nanomedicine (Oct 2019)
Salvianolic Acid B-Loaded Chitosan/hydroxyapatite Scaffolds Promotes The Repair Of Segmental Bone Defect By Angiogenesis And Osteogenesis
Abstract
Chuanlei Ji,* Long Bi,* Jing Li, Junjun Fan Department of Orthopedics Surgery, Xijing Hospital, Air Force Medical University, Xi’an 710032, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing Li; Junjun FanDepartment of Orthopedics Surgery, Xijing Hospital, Air Force Medical University, Xi’an 710032, People’s Republic of ChinaEmail [email protected]; [email protected]: Salvianolic acid B has been proven as an effective drug to promote osteogenesis and angiogenesis which could be beneficial for bone repair.Purpose: The objective of this study was to construct a salvianolic acid B-loaded chitosan/hydroxyapatite (Sal B-CS/HA) bone scaffold with controlled release and effective bioactivity.Methods: The characteristics, controlled release behavior and bioactivity of Sal B-CS/HA scaffold were evaluated in vitro. The bone repair effect was evaluated in the rabbit radius defect model.Results: The results showed that chemical and physical characteristics of salvianolic acid B and chitosan/hydroxyapatite (CS/HA) material did not obviously change after the drug loading procedure; the drug release of salvianolic acid B was stable and continuous from the Sal B-CS/HA scaffold for 8 weeks in vitro; the biocompatibility of the Sal B-CS/HA was favorable by evaluation of cell morphology and proliferation; the osteogenic and angiogenic bioactivities of the Sal B-CS/HA scaffold were proved to be effective by in vivo and in vitro tests.Conclusion: Our results suggest that this salvianolic acid B-loaded bone scaffold has potential to be used for bone defect repair with both osteogenic and angiogenic bioactivities.Keywords: angiogenesis, bone tissue engineering, controlled release, drug-loaded bone scaffold, osteogenesis, salvianolic acid B
