Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine

Felipe Lopes de Assis; Kenneth B. Hoehn; Xiaozhen Zhang; Lela Kardava; Connor D. Smith; Omar El Merhebi; Clarisa M. Buckner; Krittin Trihemasava; Wei Wang; Catherine A. Seamon; Vicky Chen; Paul Schaughency; Foo Cheung; Andrew J. Martins; Chi-I Chiang; Yuxing Li; John S. Tsang; Tae-Wook Chun; Steven H. Kleinstein; Susan Moir

Cell Reports (Jul 2023)

Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine

Felipe Lopes de Assis,
Kenneth B. Hoehn,
Xiaozhen Zhang,
Lela Kardava,
Connor D. Smith,
Omar El Merhebi,
Clarisa M. Buckner,
Krittin Trihemasava,
Wei Wang,
Catherine A. Seamon,
Vicky Chen,
Paul Schaughency,
Foo Cheung,
Andrew J. Martins,
Chi-I Chiang,
Yuxing Li,
John S. Tsang,
Tae-Wook Chun,
Steven H. Kleinstein,
Susan Moir

Affiliations

Felipe Lopes de Assis: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Kenneth B. Hoehn: Department of Pathology, Yale School of Medicine, New Haven, CT 06520, USA
Xiaozhen Zhang: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Lela Kardava: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Connor D. Smith: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Omar El Merhebi: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Clarisa M. Buckner: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Krittin Trihemasava: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Wei Wang: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Catherine A. Seamon: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Vicky Chen: Integrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Paul Schaughency: Integrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Foo Cheung: NIH Center for Human Immunology, NIAID, NIH, Bethesda, MD 20892, USA
Andrew J. Martins: Multiscale Systems Biology Section, Laboratory of Immune System Biology, NIAID, NIH, Bethesda, MD 20892, USA
Chi-I Chiang: Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA
Yuxing Li: Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Microbiology and Immunology and Center for Biomolecular Therapeutics, University of Maryland School of Medicine, Baltimore, MD 21201, USA
John S. Tsang: NIH Center for Human Immunology, NIAID, NIH, Bethesda, MD 20892, USA; Multiscale Systems Biology Section, Laboratory of Immune System Biology, NIAID, NIH, Bethesda, MD 20892, USA
Tae-Wook Chun: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Steven H. Kleinstein: Department of Pathology, Yale School of Medicine, New Haven, CT 06520, USA; Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT 06511, USA; Department of Immunobiology, Yale School of Medicine, New Haven, CT 06520, USA
Susan Moir: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding author

Journal volume & issue: Vol. 42, no. 7
p. 112780

Abstract

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Summary: Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike+ (S-2P+) and S-2P− B cells reveal clonal expansion and accumulating mutations among S-2P+ cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3+ MBCs. One branch leads to CD11c+ atypical MBCs while the other develops from CD71+ activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P+ clones, several are populated with plasmablasts at early timepoints as well as CD71+ activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs.

CP: Immunology

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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