A visualization reporter system for characterizing antibiotic biosynthetic gene clusters expression with high-sensitivity

Xiang Liu; Jine Li; Yue Li; Junyue Li; Huiying Sun; Jiazhen Zheng; Jihui Zhang; Huarong Tan

doi:10.1038/s42003-022-03832-9

Communications Biology (Sep 2022)

A visualization reporter system for characterizing antibiotic biosynthetic gene clusters expression with high-sensitivity

Xiang Liu,
Jine Li,
Yue Li,
Junyue Li,
Huiying Sun,
Jiazhen Zheng,
Jihui Zhang,
Huarong Tan

Affiliations

Xiang Liu: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Jine Li: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Yue Li: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Junyue Li: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Huiying Sun: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Jiazhen Zheng: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Jihui Zhang: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Huarong Tan: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s42003-022-03832-9
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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A quorum sensing based visualization reporter system is presented for the characterization of promoters in Gram-positive bacteria, utilizing violacein production, especially for use in the identification of secondary metabolites.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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