Metabolites (Oct 2022)

Expression Profiles of Kidney Mitochondrial Proteome during the Progression of the Unilateral Ureteral Obstruction: Focus on Energy Metabolism Adaptions

  • Ariadna Jazmín Ortega-Lozano,
  • Alexis Paulina Jiménez-Uribe,
  • Ana Karina Aranda-Rivera,
  • Leopoldo Gómez-Caudillo,
  • Emmanuel Ríos-Castro,
  • Edilia Tapia,
  • Belen Bellido,
  • Omar Emiliano Aparicio-Trejo,
  • Laura Gabriela Sánchez-Lozada,
  • José Pedraza-Chaverri

DOI
https://doi.org/10.3390/metabo12100936
Journal volume & issue
Vol. 12, no. 10
p. 936

Abstract

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Kidney diseases encompass many pathologies, including obstructive nephropathy (ON), a common clinical condition caused by different etiologies such as urolithiasis, prostatic hyperplasia in males, tumors, congenital stenosis, and others. Unilateral ureteral obstruction (UUO) in rodents is an experimental model widely used to explore the pathophysiology of ON, replicating vascular alterations, tubular atrophy, inflammation, and fibrosis development. In addition, due to the kidney’s high energetic demand, mitochondrial function has gained great attention, as morphological and functional alterations have been demonstrated in kidney diseases. Here we explore the kidney mitochondrial proteome differences during a time course of 7, 14, and 21 days after the UUO in rats, revealing changes in proteins involved in three main metabolic pathways, oxidative phosphorylation (OXPHOS), the tricarboxylic acid cycle (TCA), and the fatty acid (FA) metabolism, all of them related to bioenergetics. Our results provide new insight into the mechanisms involved in metabolic adaptations triggered by the alterations in kidney mitochondrial proteome during the ON.

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